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Premise The ability to sequence genome‐scale data from herbarium specimens would allow for the economical development of data sets with broad taxonomic and geographic sampling that would otherwise not be possible. Here, we evaluate the utility of a basic double‐digest restriction site–associated
DNA sequencing (ddRAD seq) protocol usingDNA s from four genera extracted from both silica‐dried and herbarium tissue.Methods DNA s fromDraba ,Boechera ,Solidago , andIlex were processed with a ddRAD seq protocol. The effects ofDNA degradation, taxon, and specimen age were assessed.Results Although taxon, preservation method, and specimen age affected data recovery, large phylogenetically informative data sets were obtained from the majority of samples.
Discussion These results suggest that herbarium samples can be incorporated into dd
RAD seq project designs, and that specimen age can be used as a rapid on‐site guide for sample choice. The detailed protocol we provide will allow users to pursue herbarium‐based ddRAD seq projects that minimize the expenses associated with fieldwork and sample evaluation.
